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Part:BBa_K649202:Design

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)

PlacIQ-lox71-rfp-lox66-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 686
    Illegal AgeI site found at 798
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1700


Design Notes

PlacIQ + lox2272 + RBS + mRFP + T7 terminator + lox2272 + RBS + GFP + T7

Source

The forward lox2272 is synthetic oligo and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below.

References

Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.